| Abstract |
This research article serves as a comprehensive guide for optimizing fish handling protocols during drug testing, delineating essential do's and don'ts to enhance the reliability and ethical conduct of experimental investigations. Drawing on an exhaustive literature review and expert recommendations, the manuscript systematically addresses key factors influencing fish welfare and experimental validity. The exploration encompasses temperature management, emphasizing the critical role of controlling water temperature to mitigate stress and avoid anaesthetic-induced hypoxia. pH considerations in immersion anaesthetic solutions are discussed, underscoring the importance of using buffering agents to neutralize acidity and prevent interference with drug absorption. The impact of nitrogenous compounds on gill morphology and potential methemoglobinemia is explored, advocating for careful monitoring and mitigation. Precision in drug concentration and dosage is emphasized as a crucial aspect, with a nuanced understanding of the non-linear relationships and potential tissue accumulation of certain drugs. By amalgamating practical insights and referencing pertinent literature, this article aims to provide researchers with a valuable resource, promoting the welfare of aquatic subjects and fortifying the scientific rigor of drug testing studies. Ultimately, adherence to these guidelines contributes to the refinement of drug testing protocols, fostering an ethical and reliable utilization of fish models in pharmaceutical research. |
